Молекулярная и генетическая медицина

Association Between the Angiotensinogen Gene Polymorphism and The Pro-Oxidant Effect in Tunisian Patients with Coronary Heart Disease

Abstract

Dandana A, Khelifa SB, Bouzidi N, Addad F and Ferchichi S

Background: Evaluation and investigation of the pro-oxidant role of the angiotensinogen (AGT) among Tunisian coronary.

Materials and methods: The current study evaluated the frequency of AGT (M235T and A(-6)G) polymorphism in relation to coronary artery disease in a group of Tunisian population. AGT polymorphism was determined by Real Time PCR. These subjects (150 patients and 120 controls) beneficed also by an enzymatic determination of superoxide dismutase (SOD), glutathione peroxidase (GPX), total antioxidant status (TAS), catalase (CAT) activity was determined spectrophotometrically and serum lipid peroxides were measured by the fluorimetric method reaction to reveal the atherogenic effects of these radical species and investigate their interactions with AGT polymorphisms.

Results: The results revealed that there was a positive risk of developing coronary artery disease when having the T allele whether in homozygous or heterozygous state. Statistical tests of the baseline correlation between oxidative stress parameters and M235T genotype groups showed a negative correlation between MM genotype group and oxidant parameters (SOD, GPX and TAS). The same result was found in TT and MT genotype groups.

Conclusion: This study shows that the AGT polymorphisms were associated with the presence of coronary artery disease. In addition to its vasoconstrictor role, AGT can be considered as a pro-oxidant, these two effects combine in the genesis and the complications of cardiovascular diseases.