Journal of Bioanalysis & Biomedicine

Characterization of Lipids in Femoral Atheroma from Diabetic Patients and Their Use as Clinical Descriptors

Abstract

Mónica Narváez-Rivas, Iza-Fernanda Pérez-Ramírez, Emerenciana Gallardo, José Luis González-Peña, Javier Juárez-Becerra and Manuel León-Camacho

In this work, a solid phase extraction (SPE) method is used to separate the different lipid fractions of atheroma plaque from diabetic human patients for their subsequent analysis. Sixteen fatty acids, seventeen triacylglycerols, eleven diacylglycerols being each of them as 1,2- and 1,3-isomers, two free fatty acids, 1-monoolein and seven phospholipid classes were identified. The discriminating power of the different compounds characterized has been studied in order to use them as clinical descriptor. Linear Discriminant Analysis has been applied for such purposed, diacylglycerols being the most useful compounds giving models with a total classification when sex, dyslipidemia, heart attack and stage of atheroma from patients were considered. This methodology has been used to highlight the differences between sex and age of the patients, as well as to find out differences between patients with different diseases such as dyslipidemia, heart attack, metabolic syndrome and renal failure.