Молекулярная биология: открытый доступ

CRISPR CAS9 Telomere Extension for Age-Related Diseases and Organism Longevity

Abstract

Naoki Sato

Telomere abrasion is thought to be a sign that the body is getting older. In vitro research has made significant progress in understanding the underlying biology of telomere function; however, the translation of this data to an in vivo perspective is restricted. Although there are a variety of ways for labelling telomeres, the majority of them are toxic to cells and induce DNA damage, or are incompatible with in vivo applications. By attaching Cas9 to a fluorescent protein, the CRISPR-Cas system has permitted the refinement of these areas, allowing telomeres to be visualised in living organisms. The CRISPR Cas 9 technique's high success rate offers new hope for future genome editing therapies.