Экологическая и аналитическая токсикология

Cyromazine and Chlorpyrifos Induced Renal Toxicity in Rats: The Ameliorated Effects of Green Tea Extract

Abstract

Tarek M. Heikal,Abdel-Tawab H. Mossa*,Gehan I. Kh. Marei,Mona A. Abdel Rasoul

In the present study, the protective effect of an aqueous extract of green tea (GT) against renal oxidative damage and nephrotoxicity induced by cyromazine (Cyr), chlorpyrifos (CPF) and their combination in male rats was undertaken. Eight groups containing six rats each were selected. Group I served as control. Groups II, III and IV rats were given a single daily oral doses of Cyr (169.35 mg kg-1, 1/20 LD50, in corn oil), CPF (6.75 mg kg-1 kg-1,1/20 LD50, in corn oil) and their combination for 28 consecutive days, respectively. Group V permitted free access to solubilised GT (1.5%w/v in water) as the sole drinking fluid. Groups VI, VII and VIII rats were given the same doses as groups II, III and IV and simultaneously permitted free access to solubilised GT as the sole drinking fluid. Significant reduction in body weight and elevation in kidney weight were observed in insecticides exposed rats compared to control. Significant perturbations of renal function as evidenced via increase in blood urea nitrogen (BUN) and serum creatinine level were observed in treated rats. Also, renal oxidative damage was observed in insecticide-treated rats as evidenced via augmentation in kidney lipid peroxidation (LPO) as well as depletion in kidney antioxidant enzymes; catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx). Histopathological analysis of the kidney revealed that supplementation with GT resulted in nil to mild in vacuolization, swelling and degeneration in the endothelium of glomerular tuft and the epithelium of lining tubules. In conclusion, the use of green tea extract appeared to be beneficial to rats, to a great extent by attenuating and restoring the damage sustained by insecticide exposure.