Журнал генетики и геномов

Effect of Mutations in Highly Pathogenic Avian Influenza H5N1 Viruses on the Isolation in Different Cell Culture in Comparison with Newly Circulated H5N8 Viruses in Egypt

Abstract

Azab AA, Azhar G Shalaby and Selim A

HPAIV H5N1 was circulated in Egypt since 2006 with recent introduction of H5N8 virus at the end of 2016. AIV has special virus Tropism and infectivity in different isolation host. There for we study the growth kinetics for 9 H5N1 viruses and one H5N8 virus in 3 different tissue culture systems (MDCK, CEF and VERO) and detection the potential bounding to mammalian host. Growth kinetics of cell culture growing viruses was estimated by different parameter as TCID50, CPE percent and HA activity of viruses. Growth kinetics level of Clade 2.2.1.2 and new isolated H5N8 is the highest with early detection of the virus in cell within 1st 24 hr in comparison to another 2 clades of H5N1 viruses. MDCK support high growth kinetics for all clades of virus between 3 types of cell. Antigenic characterization of the viruses supported the results of the phylogenetic analysis. Multiple peculiar mutations were characterized in the Egyptian H5N1 and H5N8 viruses. The Egyptian AI viruses preferentially bound to avian-like receptors rather than human-like receptors. MDCK cell line was suitable isolation host to replace egg isolation system with antigenic and genetic stability. This study describing the growth kinetics of HPAIV H5N1 and H5N8 in various cell lines is an informative study to understand influenza virus establishment and evolution in regard to preventive measures and cure.