Медицинская микробиология и диагностика

Effect of Some Medical Plant Extracts on Metabolism of Leishmania tropica Promastigotes In vitro

Abstract

Saeed BQ, Hassan HF and Arteen HI

The study included the inhibition effect of Equisetum arvense and Urtica piluifera extracts dissolved in cold and hot water, on the growth of Leishmania tropica. promastigotes, and the effect of these extract on metabolism activates (Total protein, carbohydrates and nucleic acids) of Leishmania tropica. Promastigotes. The inhibitory concentration of 50% of the promastigotes (IC50) at the log phase (96) hrs was 1.5 μg/ml of Equisetum arvense and 1.5 μg/ml of Urtica piluifera extracts dissolved in cold and hot water. The results revealed, that these extracts were studied has inhibitory effect L. tropica. promastigotes number, the number of L. tropica. reduced gradually when using 0.5 to 2.5 μg/ml concentrations of extracts. Moreover, these extracts were studied has inhibitory effect on total Proteins, Carbohydrates and Nucleic acids. The results of chemical analysis of the plants has highly effect on total protein, and lees effect on total carbohydrates of cell membrane, Moreover, the extract was effect on total Nucleic acids of Leishmania tropica. promastigotes after 96 hrs of cultivation.