Журнал иммунобиологии

Immunological Study for Helicobacter pylori Bacteria in Human

Abstract

Samuel Tanas Melek*, Mohamed H Yassin, Mohamed E Rashed and Taghreed A Zidan

Helicobacter pylorus is one of the most important human pathogens, infecting more than 50% of the human population. Commonly the first noninvasively testes used for H. pylori infection's detection were immunological tests like blood antibody test and stool antigen test. We investigated the more efficient susceptibility immunological test for detection of H.pylori infection in adult Egyptian patients by comparing the results of H. pylori IgA (HpIgA), IgG in serum blood (HpIgG) and H.pylori Ag in stool (HpSAg) for 30 adult patient (males and females) against control group using ELISA technique.The results showed that each test could be used successfully for diagnosis of H. pylori with 100% specificity and PPV% with no gender relation. Finally HpSAg showed reliable results with high sensitivity (>95%) followed by HpIgG (87.5%), while HpIgA showed the lowest sensitivity (37.5%). Our findings confirms the use of the mentioned immunological tests for detectingthe H. pylori infection and suggest the use H. pylori Ag in stool as the most economic, sensitive and reliable method alone or followed by IgG antibody test as confirmatory test to be the first choices for early diagnosis of H. pylori especially in developing countries.