Молекулярная биология: открытый доступ

Malt Genotypic Screening of Polymorphism Information Content (PIC) of SSR Markers Based on Physiological Traits in Barley

Abstract

Gang Qian, Junjiao Ping, Dazhong Wang, Zhen Zhang, Suyuan Luo and Mingzhen Yang

Brewing genotypes were described by polymorphism information content (PIC) of 22 SSR markers in barley (Hordeum vulgare L.), associated with malt quality. Notably, the values of diastatic power set ranged from 5.53 to 24.13 U/Kg in these samples, suitable to assess the relative importance of biochemical components of malt variations and genetic characteristics. Cluster analysis revealed a significant correlation between eco-geographic origin and SSR marker clustering, resolving the accessions into four subgroups. Moreover, SSRs with higher PIC values and higher average number of alleles per marker in the population with higher values of diastatic power were found to be an efficient utility for distinguishing brewing genotypes in barley. These results possibly indicated that SSRs linked to brewing traits could be very useful for application in monitoring malt traits, evaluating genetic diversity, and determining the sampled eco-geographic origins in barley.