Ветеринарная наука и технологии

Phylogenetic Analysis of Toxoplasma gondii Type II and Type III by PCRRFLP Plus Sequencing on Wild-Rats of Golestan Forest, Iran

Abstract

Ehsan Shariat Bahadori, Javid Sadraei, Abdolhosein Dalimi, Somayyeh Namroodi and Majid Pirestani

Introduction: Toxoplasma is a genus of intracellular parasites, which was observed in the many types of mammalians. Toxoplasma protozoa that were detected from many types of rats have the equal phenotypic characteristics but have different clinical symptoms and genotypes. Mostly, domestic animals and rodents are the hosts in the reservoirs in the environment. The objective of this article was to phylogenetic identification of toxoplasmosis in wild-rats of Golestan forest using secretory antigens type 1 gene. Materials and Methods: In this surveillance, the researchers gathered 286 wild rodents from Golestan forest and earned DNA from brain and heart tissues to prove SAG1 gene from these hosts. They split these rodents into four groups and then analyzed the positive samples by the Polymerase chain reaction basis. Results: In this surveillance, they discovered 24% of tissues of the wild-rats were infected for these protozoa. Over 55.8% of tissues belonged to Ratus ratus, 17.6% tissues belonged to Ratus norvegicus, 14.7% of tissues belonged to Mus musculus, 11.7% of tissues belonged to Rombumys opimus, also with attention of phylogenetic analysis, 50 infected rats belonged to genotype ΙΙΙ and 18 infected rats belonged to genotype ΙΙ. Conclusion and Discussion: Conclusion showed that different types of wild rats disseminated this type of infection; also SAG1 molecule was a very important molecule to detect toxoplasmosis in rats of Golestan area.