Ветеринарная наука и технологии

Semen Discarded During Different Stages of Cryopreservation in Ongole (Bos indicus) Bulls

Abstract

Manda Srinivas, Makkena Sreenu, Srilatha CH, Babu Rao K and Naidu KS

Indian subcontinent is a treasure house of Bos indicus cattle, one of the most popular breed amongst them is “Ongole” which by inheritance is a dual purpose breed. During the period under report 549 ejaculates were collected from eight breeding bulls, of which 192 ejaculates (34.97%) were discarded during various stages of semen processing. Out of the total ejaculates, 126 (22.95%), 40 (7.29%) and 26 ejaculates (4.74%) were discarded after collection, equilibration and freezing, respectively while most of the discards were second ejaculates. From the present study, it was concluded that the ejaculate discard in Ongole bulls was mainly based on low volume, initial progressive motility, sperm concentration and post-thaw motility. However, it was suggested that certain reproductive technologies like Sephadex or Glass wool filtration or Percoll gradients could be used to enhance the quality of these low grade ejaculates from genetically superior bulls.