Журнал биомедицинских систем и новых технологий

Transdermal Drug Delivery of Adenosine by Microplasma with Iontophoresis and DMSO

Abstract

Jaroslav Kristof1*, Marius Blajan1, Ryo Yokoyama2, Fariha Mustafa2, Ahmad Guji Yahaya3 and Kazuo Shimizu1,2,3

Medication brings negative effects regarding the method of the drug administration, such as risk of infections and metabolism of drugs. Transdermal drug delivery can avoid these problems. Microplasma discharge treatment was used to overcome the skin barrier. TEM observation of the untreated and microplasma treated skin confirmed the effect of microplasma on lipid lamellae. Franz diffusion cell was used to investigate permeation of Adenosine through Yucatan micropig skin. The amount of the penetrated drug and the drug retained inside the skin was measured by HPLC. We compared Adenosine permeation through the untreated skin, iontophoreticaly pre-treated skin with FeSO₄�??7H₂O, iontophoreticaly pre-treated skin with FeSO₄�??7H₂O followed by microplasma treatment, and DMSO treated skin. Iontophoretic pre-treatment with FeSO₄�??7H₂O before microplasma treatment was used with idea to enhance a lipid oxidation inside the skin. Microplasma treatment was the most effective in permeation of Adenosine. On the other hand, DMSO treatment and iontophoreticaly pre-treated skin followed by microplasma treatment retained the most and the similar amount of the drug inside the skin.