Журнал биопереработки и биотехники

Optimization of Cellulase Production from Newly Isolated Bacillus sp. Y3

Abstract

Yogita Lugani, Rajesh Singla and Balwinder Singh Sooch*

Cellulose, a major constituent of plant cell wall, is the most abundant biological polymer on earth. The use of various cellulolytic microorganisms for the bioconversion of cellulose into value added products has attracted a worldwide attention. Hence the present work was aimed to isolate new cellulase producing microorganisms and further to investigate the effect of nutritional and process parameters on cellulase production from selected isolated culture. Out of 20 cellulase producing bacterial strains isolated during the study, Y3 isolate was found to be best for the production of cellulase enzyme. This isolate was then characterized for its morphological and biochemical characters and identified as Bacillus sp. Y3. The effect of different parameters like carbon sources, nitrogen sources, temperature, pH, inoculum concentration and incubation time was monitored with selected strain for cellulase production. The maximum FPase and CMCase activity of Bacillus sp. Y3 was 6.84 IU/mL and 7.82 IU/ mL, respectively, when the basal media of pH 7 containing CMC (1%, w/v) and peptone (1%, w/v) was inoculated with 2% (v/v) inoculum and incubated at 37°C for 96 hours at 120 rpm. The FPase (6.84 IU/mL) and CMCase activity (7.82 IU/mL) obtained after optimization was much higher than FPase (1.97 IU/mL) and CMCase activity (2.48 IU/ mL) before optimization.