Журнал биоразнообразия и исчезающих видов

The amount and quality of DNA produced, as well as its appropriateness for subsequent PCR amplifications, varied significantly depending on the DNA extraction technique used. Quantitative PCR enumeration of microbial marker loci was used to further evaluate DNA extracts from nine extraction techniques that satisfied these initial quality standards. Depending on the DNA extraction technique utilised, the same rumen samples' absolute bacteria counts varied by more than 100-fold. Using 454 Titanium pyro sequencing, the apparent compositions of the archaeal, bacterial, ciliate protozoal, and fungal communities in similar rumen samples were evaluated.

In order to mitigate the effects of climate change and secure global food production, plant breeding is crucial in addition to crop management and policy changes. The goals, effectiveness, and genetic gains of the existing plant breeding system, however, are also impacted by changes in environmental conditions. In this study, we discuss the difficulties associated with breeding climate-resilient crops as well as the limitations of the next-generation breeding strategy. Crops that are prepared for climate change should be available sooner thanks to the integration of multiple disciplines and technology into the three schemes of genotyping, phenotyping and envirotyping.

In general, the phrase "biodiversity" refers to variation at the molecular level in living things. It is a laborious procedure to gather scientific data on biodiversity, and it is currently difficult to provide proper data storage, maintenance, and interchange on a national and international scale. We require digitised, structured, and validated biodiversity data in order to accurately assess the status of biodiversity for the purposes of sound decision-making and management of biodiversity and ecosystems. Mechanisms for exchanging data, standards, and experiences must also be established. This can be done by reviewing and putting into practise regionally agreed-upon technical and biodiversity standards for data interchange, species and habitat lists, as well as by maintaining constant dialogue, coordination, and communication among all pertinent parties.