Журнал генетики и исследований ДНК

Panax Notoginsenosides Attenuates Inflammation in Rabbit Model

Abstract

Rahim Abdul

Panax Notoginsenosides (PNS) performs the function of enhancing blood circulation. The aim of this study is to investigate the effect of intrapleural PNS on rabbit pleural inflammation reaction, and determine the levels of transforming growth factor β1 (TGF-β1) and Vascular Endothelial Growth Factor (VEGF). Forty New Zealand white rabbits were divided into four groups. The rabbit pleural inflammation reaction model was established by injection of tetracycline hydrochloride solution into pleural cavity. Then PNS, urokinase (UK) and PBS were injected into the pleural cavity as experiment groups. While tetracycline hydrochloride solution was replaced by phosphate buffer solution (PBS) as control group. The pleural effusion was collected at 24 h, 48 h, 72 h and 96 h in all groups, and then biochemical indicators, TGF-β1 and VEGF were detected. On day 14, all animals were killed and pleural tissues were collected to perform hematoxylin eosin (HE) and Masson trichrome staining. The results indicated that levels of TGF-β1 and VEGF were significantly lower in PNS group than that in UK group and PBS group (Pand inhibit collagen production, which had better effects compared with UK, single gene conferring resistance to P. neglectus, Rlnn1, has been mapped to chromosome 7AL. QTL analysis in several bi-parental mapping populations has identified major QTL for P. thornei resistance on chromosomes 2BS, 6DS and 7BL, which have been verified in sources of resistance from diverse backgrounds. Genotyping-by-sequencing has provided closely linked flanking markers that are now available to Australian breeders through the Australian Wheat and Barley Program to implement marker-assisted selection. Further fine mapping using large segregating populations will allow map-based cloning approaches to identify candidate genes underlying these QTL for RLN reon reaction model was established by injection of tetracycline hydrochloride solution into pleural cavity. Then PNS, urokinase (UK) and PBS were injected into the pleural cavity as experiment groups. While tetracycline hydrochloride solution was replaced by phosphate buffer solution (PBS) as control group. The pleural effusion was collected at 24 h, 48 h, 72 h and 96 h in all groups, and then biochemical indicators, TGF-β1 and VEGF were detected. On day 14, all animals were killed and pleural tissues were collected to perform hematoxylin eosin (HE) and Masson trichrome staining. The results indicated that levels of TGF-β1 and VEGF were significantly lower in PNS group than that in UK group and PBS group (Pand inhibit collagen production, which had better effects compared with UK, single gene conferring resistance to P. neglectus, Rlnn1, has been mapped to chromosome 7AL. QTL analysis in several bi-parental mapping populations has identified major QTL for P. thornei resistance on chromosomes 2BS, 6DS and 7BL, which have been verified in sources of resistance from diverse backgrounds. Genotyping-by-sequencing has provided closely linked flanking markers that are now available to Australian breeders through the Australian Wheat and Barley Program to implement marker-assisted selection. Further fine mapping using large segregating populations will allow map-based cloning approaches to identify candidate genes underlying these QTL for RLN